WebRQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The … WebThe RQ1 DNase digestion capacity was demonstrated by the treatment of free CpG-ODN (line 6). BSA in the formulation did not affect the CpG-ODN protection (lines 9 and 10). CpG-ODN, CpG-oligodeoxynucleotides; BSA, bovine serum albumin; SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis.

Rq1 rnase-free dnase Sigma-Aldrich

WebRQ1 (RNA-Qualified) RNase-Free DNase is a DNase I that degrades both double- stranded and single-stranded DNA endonucleolytically, producing 3´-OH oligonu- cleotides (1). … WebRQ1 DNase 5 μL: 93. Incubate the mixture for 20 min at 37°C. This DNase step reduces the production of spurious PCR products in subsequent amplification rounds and ensures that amplicons are derived from RNA by RT–PCR. 94. Extract the RNA as follows: To the DNase reaction, add 300 μL ... chinees goes

Thermostabilization and thermoactivation of thermolabile enzymes ... - PNAS

WebRQ1 RNase-Free DNaseは、高純度に精製されたDNase I 製品で、一本鎖あるいは二本鎖DNAを分解して3'-末端に水酸基を持つオリゴヌクレオチドを生成します。 特にRNAの … WebRQ1 DNase reaction buffer. 400 mM Tris (pH 8.0) 100 mM MgSO 4. 10 mM CaCl 2. Aliquot (1 μL of buffer is used per reaction) and store frozen. WebRQ1 RNase-Free DNase. Catalog Number: (PAM6101) Supplier: Promega Corporation. Description: RQ1 (RNA Qualified) RNase-Free DNase is a DNase I that degrades both double-stranded and signle-stranded DNA. Certificates. Retrieving Each-+ Alternative Product for Part Number 04716728001 ... chinees goor wing wah